Adipose Gene Expression Profiles Related to Metabolic Syndrome Using Microarray Analyses in Two Different Models
Diabetes & Metabolism Journal 2014³â 38±Ç 5È£ p.356 ~ p.365
·ùÇýÁø(Yoo Hye-Jin) - Korea University College of Medicine Department of Internal Medicine
ȲȯÁø(Hwang Hwan-Jin) - Korea University College of Medicine Department of Internal Medicine
Á¤Å¿ì(Jung Tae-Woo) - Korea University College of Medicine Department of Internal Medicine
·ùÀÚ¿µ(Ryu Ja-Young) - Korea University College of Medicine Department of Internal Medicine
ȫȣö(Hong Ho-Cheol) - Korea University College of Medicine Department of Internal Medicine
ÃÖÇýÀ±(Choi Hae-Yoon) - Korea University College of Medicine Department of Internal Medicine
¹é¼¼Çö(Baik Sei-Hyun) - Korea University College of Medicine Department of Internal Medicine
Ãְ湬(Choi Kyung-Mook) - Korea University College of Medicine Department of Internal Medicine
Abstract
Background: Peroxisome proliferator-activated receptor-¥ã (PPAR-¥ã) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-¥ã agonists and increased in OLETF rats using microarrays in two different models.
Methods: In the first microarray analysis, PPAR-¥ã agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats).
Results: Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-¥ã agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count.
Conclusion: Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-¥ã agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers.
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Lipocalin-2, Microarray, PPAR gamma
KMID :
1200020140380050356
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